Background and Mechanism of saRNA
Self-amplifying mRNA (saRNA) molecule simultaneously encodes both the therapeutic payload(s) and an RNA replicase derived from alphavirus. Once saRNA is inside the cell, the rapidly expressed RNA replicase exponentially ‘copies and pastes’ the payload-encoding RNA, ensuring efficient protein expression.
In comparison to non-replicating linear mRNA, saRNA effectively leverages the unique RNA amplification effect, resulting in:
Maximize the amount of target protein expression and increase duration
Enhance the integrity and yield
Enable independent expression of multiple payloads